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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Commodity Protection and Quality Research » Research » Research Project #443803

Research Project: Producing Sterile Navel Orangeworm on Demand for Improvement of Pest Management (Dr. Raman Bansal)

Location: Commodity Protection and Quality Research

Project Number: 2034-43000-043-054-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Mar 1, 2022
End Date: Feb 28, 2024

Objective:
The objective is to determine navel orangeworm’s molecular response to ionizing radiations.

Approach:
To determine Navel Orangeworm (NOW’s) molecular response, we will adopt genome-wide approach to analyze gene expression using high-throughput sequencing. Virgin moth cohorts will be exposed to standardized X-ray radiation dose. A subset of treated male and female will be paired immediately after the irradiation. Moths without the radiation exposure will also be paired (wild control). Wild and treated moths (four biological replicates each) will be collected 12 and 24h later. Females from mated pairs will be dissected out to collect spermatophores. Whole body and spermatophores samples will be processed for RNA-seq.