Project Number: 3091-22000-040-005-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Jan 1, 2023
End Date: Dec 31, 2023
1. Preparing for the release of nematode resistance lines fully homozygous for the Mi1, Mi2, Ren2, Ren3 markers. 2. Preparing for the release of the insect and nematode resistant lines derived by introgression of caryophyllene oxidase from triple species hybrids into BARBREN 713-32-30. 3. Develop germplasm lines with Fov4 resistance from SA-3208, the insect resistance from the triple species hybrid HAA, and reniform and root-knot nematode resistances from the BAR 32-30 breeding line. 4. Seed increase and testing of Fov and nematode resistant lines derived by introgression of Fov4 resistance from G. barbadense 'Pima S-4' and 'Pima S-6' into the BAR 32-30 line.
1. All the resistance and agronomic performance testing and seed increase have been completed for the germplasm lines with high level of resistance to root-knot and reniform nematodes that are homozygous for Mi1, Mi2, Ren2 and Ren3 markers. The release documentation for the lines will be prepared and submitted. 2. The insect resistant trait associated with the caryophyllene oxidase from triple species hybrid was introgressed into BAR32-30 resistant to reniform and root-knot nematodes. The field seed increase of the BC5 selfed progenies harvested in 2022 will be evaluated in the Reginal Breeders Testing Network in 2023. Six additional lines will be field tested for resistance and agronomic performance in 2023. 3. F3 plants were generated from the cross between SA 3208 with Fov4 resistance and the insect-resistant BC5 line derived by introgression of caryophyllene oxidase gene from triple species hybrid HAA into BAR 32-30 resistant to reniform and root-knot nematodes. Fov4 resistance will be evaluated in the greenhouse tests and insect resistance will be selected based on chemical analysis of caryophyllene derivatives. Insect resistance and agronomic performance will be field tested. These lines will also control Fov1 by suppressing nematode populations to levels below those required for Fusarium wilt. 4. Fov4 resistances from G. barbadense 'Pima S-4' and 'Pima S-6' were introgressed into the nematode resistance lines BAR-32-30. Seed of BC5S4-5 generations were increased in the greenhouse. There are 104 plants that derived from Pima S-6 and 52 plants that derived from Pima S-4. The plants selected were all homozygous for the Fov4 resistance. The harvested seed will be screened and tested in field in 2023.