Location: Crop Diseases, Pests and Genetics Research
Project Number: 2034-22000-015-002-I
Project Type: Interagency Reimbursable Agreement
Start Date: Oct 1, 2022
End Date: Aug 31, 2024
Objective:
Determine the principal vector(s) of CYVCV in the Tulare outbreak area and relevant host reservoirs and evaluate vector population dispersal.
Approach:
Insects will be collected by aspiration and established on plants in the insectary. Virus (CYVCV)-infected blind buds will be graft inoculated in citrus plants and/or mechanically inoculated in beans in a USDA-approved greenhouse facility. Virus detection will be by RT-PCR. Insect vectors will be transferred by camel-hair brush. Virus symptoms will be documented by pictures and titer measured by RT-qPCR. Insect vectors with be marked by SmartWater liquid fluorescent solution and released in the field for mark/recapture experiments. Marked insects will be deteched by UV light with stereo microscope to determine local spatial movement and dispersal of vectors.
