Location: Agroecosystem Management Research
Project Number: 3042-12630-004-006-I
Project Type: Interagency Reimbursable Agreement
Start Date: Dec 15, 2021
End Date: Sep 30, 2022
Objective:
FDA and ARS jointly agree to develop and evaluate methods for the detection (and/or quantification) of AMR in enteric bacteria in water, for the purpose of monitoring selected antibiotic resistance phenotypes and genotypes. These data will advance the NARMS environmental monitoring program objectives by compiling and developing standardized protocols for detection of Salmonella, E. coli and Enterococci from one litter and ten litter volumes of water. Dr. Durso will develop preliminary protocols for intra- and inter-laboratory methods to both enumerate and isolate E. coli and Enterococcus able to grow in the presence of preterminal antibiotic drug concentrations.
Approach:
With input and consensus from the NARMS EWG steering committee, and in collaboration with the NARMS EWG culture working group, the following methods will be evaluated for suitability of E. coli and Enterococcus targets n the NARMS Environmental Surveillance pilot projects. 1/ plating samples directly onto media with and without supplemented antibiotics for the purpose of quantification (no enrichment). 2/ Plating a non-selective enrichment onto antibiotic supplemented media (yes/no detection - not suitable for quantification). 3/ Modification of IDEXX Quantitray with and without added antibiotics for quantification.
