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ARS Home » Pacific West Area » Wapato, Washington » Temperate Tree Fruit and Vegetable Research » Research » Research Project #440411

Research Project: Applying Molecular Markers To Select For Promising Virus Resistant Germplasm

Location: Temperate Tree Fruit and Vegetable Research

Project Number: 2092-21220-003-014-T
Project Type: Trust Fund Cooperative Agreement

Start Date: May 1, 2021
End Date: Jun 30, 2024

Objective:
Objective 1: Generate KASP compatible molecular marker that can be used to screen for TRV resistance derived from Castle Russet. Objective 2: Develop shared protocols to grow out, genotype, and tag large populations segregating for dominant PVY and TRV resistance alleles. Objective 3: Validate selection of PVY and TRV resistant individuals using field trials. Objective 4: Generate PVY (multiple resistance genes) and TRV resistant material to address each marker class.

Approach:
Objective 1: Generate KASP compatible molecular marker that can be used to screen for TRV resistance derived from Castle Russet. We will submit five-to-ten 101 base pair context sequence spanning QTL peak intervals associated with TRV resistance to the LGC Biosearch Technologies ‘KASP-by-Design’ service. We will also investigate other computational approaches for primer design described in (Makhoul et al., 2020) based upon the recently completed Castle Russet genome. Objective 2: Develop shared protocols to grow out, genotype, and tag large populations segregating for dominant PVY and TRV resistance alleles. We will grow out 1000 individuals from a Castle Russet X A06084-1TE and 1000 individuals from the Eva X Redsen or Eva X NY97 cross and contract KASP genotyping with Intertek via the CGIAR Excellence in Breeding Platform. Objective 3: Validate selection of PVY and TRV resistant individuals using field trials. Grow out subsets of each population that were identified to contain the virus resistance locus and those that do not, then examine if they exhibit field immunity to PVY and TRV in disease infested fields in Hermiston, OR and Prosser, WA. Objective 4: Generate PVY (multiple resistance genes) and TRV resistant material to address each marker class. We will generate material that segregate for both PVY resistance alleles (Rysto , Ryadg) and TRV resistance derived from Castle Russet. Selection within this dual resistance PVY material will provide clones with long term resistance to PVY. We will also source breeding lines from other potato breeding programs that contain these and other alleles and add them to our crossing block. This will increase the genetic diversity within our breeding program.