Location: Commodity Protection and Quality Research
Project Number: 2034-43000-043-026-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Sep 1, 2020
End Date: Aug 31, 2024
Objective:
Determine red flour beetle’s molecular response to optimal concentrations of phosphine.
Approach:
To determine red flour beetle’s molecular response, we will adopt genome-wide approach to analyze gene expression using high-throughput sequencing. For fumigant treatment, relatively susceptible and tolerant adult populations (n = 100) will be exposed to two steady-state phosphine doses (1000 and 3000 ppmv) in sealed gas-tight glass jars at respective treatment times to cause 50% mortality (LT50s). To identify various insect genes regulated following the phosphine exposure, statistical comparison of expression values in different treatments in comparison to control will be conducted using generalized linear models.
