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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Parasitic Diseases Laboratory » Research » Research Project #437325

Research Project: Development of New Swine Reagents to Broaden Our Understanding of Immune Correlates of Protection and Microbial Pathogenesis

Location: Animal Parasitic Diseases Laboratory

Project Number: 8042-32000-102-030-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Jul 15, 2019
End Date: Jul 14, 2022

Objective:
1. Develop and characterize panels of monoclonal antibdies (mAb ) specific for swine immune proteins, e.g., cytokines. 2. Generate SLA class I tetramers for several common US pig breeds. 3. Express specific transcription factors regulating critical immune interactions and develop/identify mAbs to them. 4 Generate/identify mAbs specific for porcine CD antigens. 5. Continue to transfer developed reagents to commercial sources.

Approach:
The ARS coPI from the Animal Parasitic Diseases Laboratory will focus on Objectives 1 and 5. Objective 1 targets will include characterizing mAbs produced by commercial partners. These include mAbs anti-interferon-beta (IFNb), interleukin-3 (IL-3), IL-29, IL-31, IL-33, PD-L1, BAFF and APRIL. This will also include analyses of mAbs produced during earlier US UK swine toolkit grant. ELISAs will be used to confirm specificity of mAbs, define epitopes recognized and cross species reactivities. Intracellular staining will be tested on pig cell subsets as appropriate. For Objective 5 Hybridoma cell lines and mAbs produced during our earlier US UK swine toolkit grant as well as new mAbs will be provided to swine researchers and licensed to commercial sources.