Project Number: 2072-22000-045-005-G
Project Type: Grant
Start Date: Aug 1, 2019
End Date: Jul 31, 2024
Determine the levels of fungicide resistance in isolates of Botrytis species.
Isolates of Botrytis species in the Washington State University Puyallup culture collection will be tested for their resistance to 7 fungicides (iprodione, thiophanate-methyl, fenhexamid, boscalid, pyraclostrobin, fludioxonil, and cyprodinil). Typically, resistance testing involves comparing the growth of each isolate on media amended with various concentrations of fungicide to determine the concentration of fungicide required to inhibit the growth of each isolate on non-amended media by 50% (EC50). This requires a large amount of expendable supplies (media and petri plates) and labor. The use of a Spiral Dilution Plater provides an alternative way to determine EC50’s that require significantly less expendable supplies and labor (Förster et al. 2014).