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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Disease and Pest Management Research Unit » Research » Research Project #437077

Research Project: Management of Botrytis of Cut Flowers

Location: Horticultural Crops Disease and Pest Management Research Unit

Project Number: 2072-22000-045-005-G
Project Type: Grant

Start Date: Aug 1, 2019
End Date: Oct 31, 2022

Objective:
Determine the levels of fungicide resistance in isolates of Botrytis species.

Approach:
Isolates of Botrytis species in the Washington State University Puyallup culture collection will be tested for their resistance to 7 fungicides (iprodione, thiophanate-methyl, fenhexamid, boscalid, pyraclostrobin, fludioxonil, and cyprodinil). Typically, resistance testing involves comparing the growth of each isolate on media amended with various concentrations of fungicide to determine the concentration of fungicide required to inhibit the growth of each isolate on non-amended media by 50% (EC50). This requires a large amount of expendable supplies (media and petri plates) and labor. The use of a Spiral Dilution Plater provides an alternative way to determine EC50’s that require significantly less expendable supplies and labor (Förster et al. 2014).