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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Molecular Plant Pathology Laboratory » Research » Research Project #436115

Research Project: Emerging Stress Challenges and Functional Genomics of Stress Responses in Alfalfa

Location: Molecular Plant Pathology Laboratory

2019 Annual Report


4. Accomplishments
1. Discovered a novel species of RNA virus identified in the migratory root lesion nematode Pratylenchus penetrans. The root lesion nematode Pratylenchus penetrans is a migratory species affecting agriculturally important crops worldwide, including alfalfa. The root lesion nematode virus (RLNV) is the first virus identified in the migratory nematode species of the genus Pratylenchus. The effect of the virus infection on the viability and development of the nematodes has yet to be clarified. The research is significant because it provides insights into the nematode biology and may result in application of virus infection for pest control strategies.

2. For the first time, identified a strain of Cycas necrotic stunt virus (CNSV) in publicly available transcriptomic datasets of alfalfa. Prior to this work, the virus was not reported to infect alfalfa. Cycas necrotic stunt virus was first detected in the gymnosperm Cycas revoluta in Japan where it caused severe leaves abnormalities. The virus identified in alfalfa by global surveys of transcriptomic datasets represents a new strain of CNSV adapted to alfalfa, for which the name CNSV-A was proposed. The research is significant because the biology of this emerging virus in alfalfa is not known and requests further investigation.

3. For the first time, identified a strain of cnidium vein yellowing virus in publicly available transcriptomic datasets of alfalfa. Prior to this work, the virus was not reported in alfalfa and was only known to infect cnidium, a medicinal plant species (Cnidium officinale) in Korea, where infected cnidium plants displayed yellowing leaf symptoms. The research is significant because the biology of this emerging virus in alfalfa is not known and requests further investigation.


Review Publications
Nemchinov, L.G., Francois, S., Roumagnac, P., Hammond, R., Mollov, D.S., Filloux, D. 2018. Characterization of alfalfa virus F, a new member of the genus Marafivirus. PLoS One. 13(9):e0203477. https://doi.org/journals.plos.org/plosone/article?id=10.1371/journal.pone.0203477.
Nemchinov, L.G., Lee, M., Shao, J.Y. 2018. First report of alphapartitiviruses infecting alfalfa (Medicago sativa) in the United States. Genome Announcements. https://doi.org/mra.asm.org/content/7/21/e01266-18.
Vicente, C.S., Nemchinov, L.G., Mota, M., Eisenback, J.D., Kamo, K.K., Vieira, P. 2019. Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans. PLoS One. 14 (2):e0212540. https://doi.org/10.1371/journal.pone.0212540.
Vieira, P., Nemchinov, L.G. 2019. A novel species of RNA virus associated with root lesion nematode Pratylenchus penetrans. Journal of General Virology. https://doi.org/10.1099/jgv.0.001246#tab2.