Project Number: 2072-22000-041-020-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Oct 1, 2018
End Date: Aug 31, 2022
1. Detection and Monitoring: How to improve resistance detection and distribution assessment? 2. Predicting Resistance Spread: Where and when does it arise and how does it disperse?
1. Develop nucleic amplification assays to detect fungicide resistance in lab and/or field settings. Conduct on-site trials with growers or diagnostics labs and develop quality control best practices for assays. Using biocontainment protocols examine evolution of Succinate dehydrogenase inhibitor (SDHI) fungicide resistance in E. necator isolates. Compare inoculum monitoring techniques to rapid sampling protocols for estimating the spatial and temporal distribution of fungicide resistance. Rapid sampling kits will be given to growers across the U.S., and used for evaluating the presence of genes related to Demethylation inhibitor (DMI) and Quinone outside inhibitors (QoI) resistance. 2. Evaluate grower spray records to determine the relationship of fungicide use and onset or suspicion of fungicide resistance. This will be used to model (and subsequently validate) fungicide resistance application thresholds. Use quasi-physical transport models coupled to Weather Research and Forecasting (WRF) model and resistance monitoring data to field/farm/subregion scale dispersion models to estimate source strength to construct regional resistance risk. Conduct seasonal monitoring and recurring collection of isolates from selected grower vineyards for Azanaphthalene (AzN), DMI, QoI and SDHI resistance and genetic diversity.