ARS | Publication request: GENETIC MAPPING OF MUSKMELON (CUCUMIS MELO L.) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHIC (AFLP) MARKERS

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: August 10, 1997
Publication Date: N/A

Technical Abstract: Muskmelon (Cucumis melo L.) has 12 chromosomes (2n=2x=24) with a genome size of 5 x 10(8th) bp. A genetic map with sufficient markers is not yet available. For construction of a map with higher density we identified Amplified Fragment Length Polymorphic (AFLP) markers. The mapping population consisted of sixty-six backcross progenies derived from MR-1 (a breeding line with multiple disease resistance) and Ananas Yokneam (AY, an Israeli landrace susceptible to many diseases) with AY as recurrent parents. Only markers present in MR-1 were included in the map. Initial screening of parents identified 429 markers, but only 221 (together with RAPD markers) were scored in the progeny. One hundred eighty-eight of the markers fell into 14 linkage groups with 6-28 markers in each group using a lod score of 4 and maximum theta of 0.25. The map covers over 1942 cM. Several markers linked to Fom-2, a gene for resistance to Fusarium wilt, were identified.