Skip to main content
ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #72205
Title: WIDE CROSSES FOR HAPLOIDS

Author
item Rines, Howard
item RIERA-LIZARAZU, OSCAR - UNIVERSITY OF MINNESOTA
item MAQUIEIRA, SILVIA - UNIVERSITY OF MINNESOTA
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: Oat International Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 8/6/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The objective of this paper is to review findings on haploid production via wide crosses in cereals with an emphasis on the very wide crosses (across subfamilies) and in particular on some novel and exciting results we have obtained from crosses of oat (Avena sativa L.) by maize (Zea mays L.). The initial consistent production of cereal haploids via wide hybridization was sfrom an interspecific cross, with barley (Hordeum vulgare L.) haploid plan recovery following crosses with H. bulbosum L. The discovery that H. bulbosum pollinations of certain wheat (Triticum aestivum L.) cultivars enabled the production of wheat haploids by chromosome elimination raised hopes for increased use of doubled haploids in wheat breeding. An exciting alternative to H. bulbosum as pollen donor for wheat haploid production came from the description of haploid wheat embryos being produced from wheat x maize hybridizations by elimination of the maize chromosomes during gthe initial cell divisions of the developing embryo. Cultivated hexaploid oat was found to be like wheat in yielding haploid plants through uniparental pollen donor chromosome elimination following inter-subfamily hybridization with maize. Haploid oat plants are unique in that they may be partially self-fertile through the production of unreduced gametes. Another novel feature arising from oat x maize hybridization is that we have found pollen donor chromosome retention to be relatively common in oat x maize crosses. Fertile maize chromosome addition oat lines have been recovered for maize chromosomes 2, 3, 4, 7, 8, and 9. These plants represent novel materials for maize gene mapping and isolation, study of maize gene expression in oat, and potential introgression of maize DNA into oat.