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United States Department of Agriculture

Agricultural Research Service

Title: Quantification of Immunoglobulin in the Serum and Mucus of Channel Catfish at Different Ages

item Zilberg, Dina - AUBURN UNIVERSITY
item Klesius, Phillip

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: March 4, 1997
Publication Date: N/A

Technical Abstract: An immunoassay was developed to quantify immunoglobulin (Ig) in the serum and mucus of channel catfish. The Falcon Assay Screening Test (FAST) enzyme-linked immunosorborbent assay (ELISA) beads were sensitized with serum or mucus samples and their Ig levels quantified with goat anti-catfish Ig and mouse anti-goat Ig conjugate. The minimum detectable level of serum Ig was 0.1 ug m1. The assay quantified serum Ig from 0.15 to 6.0 ug Ig m1. Coefficients of intra- and interassay variations of 1.7 to 12.4% and 1.5 to 10.2% respectively, indicated that the assay was highly reproducible. Serum Ig concentration was found to increase from 1.44 0.56 to 42.3 48.43 mg m1 between 3 and 15 months of age. In contrast, mucus Ig concentration slightly decreased from 0.016 ug cm of fish skin between 3 and 15 months of age. Serum Ig was apparently not transduced into the mucus of channel catfish.

Last Modified: 4/18/2015
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