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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #57738
Title: DISOMIC MAIZE CHROMOSOME-ADDITION OAT PLANTS DERIVED FROM OAT X MAIZE CROSSES

Author
item Rines, Howard
item RIERA-LIZARAZU, OSCAR - UNIVERSITY OF MINNESOTA
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: Modification of Gene Expression and Non-Mendelian Inheritance Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 3/30/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: In haploid plants recovered from oat (Avena sativa L.) x maize (Zea mays L.) crosses, about one-third (30 of 90) retained one to four maize chromosomes in addition to a haploid set of 21 oat chromosomes. Oat haploids with one or two maize chromosomes were partially self-fertile by a meiotic restitution process and showed nearly complete transmission of the maize chromosome(s) to give disomic maize-chromosome addition oat lines. Restriction fragment length polymorphisms (RFLPs) were used to identify the maize chromosome(s) present in the various addition lines. Disomic addition lines (21 pairs of oat plus 1 pair of maize chromosomes) have been identified for five of the ten maize chromosomes--one line carried maize chromosome 2, one with chromosome 3, four with chromosome 4, two with chromosome 8, and two with chromosome 9. A double disomic addition line carried maize chromosomes 4 and 7. Also, maize chromosomes 5 and 6 have been identified as additions in haploid lines. The maize chromosome-addition lines of oat represent unique, exciting materials for investigations including testing for expression of maize genes in oat analyzed through RNA and protein patterns, new traits such as disease resistance added to oat, modifications in maize chromatin structure, intergeneric translocations, and transfer of active transposable elements from maize to oat.