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United States Department of Agriculture

Agricultural Research Service

Research Project: Innovative Bioresource Management Technologies for Enhanced Environmental Quality and Value Optimization

Location: Coastal Plain Soil, Water and Plant Conservation Research

Title: Enhanced deammonification of livestock wastewater using Brocadia caroliniensis and HPNS in single tank process

item Vanotti, Matias
item Nishiyama, Takaski -
item Martinez, Jose -
item Kunz, Airton -
item Fujii, Takao -
item Furujawa, Kenji -

Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: May 10, 2013
Publication Date: June 11, 2013
Citation: Vanotti, M.B., Nishiyama, T., Martinez, J., Kunz, A., Fujii, T., Furujawa, K. 2013. Enhanced deammonification of livestock wastewater using Brocadia caroliniensis and HPNS in single tank process. In: Proceedings of the 2nd International Anammox Symposium, June 11-12, 2013, Coex, Seoul, Korea, p. 39-40.

Technical Abstract: In this work we describe new findings that allowed rapid implementation of deammonification reaction in livestock anaerobic digestion effluents using mixtures of two bacterial cultures and a one-stage process (partial nitritation and anammox in a single tank). The bacterial cultures were high performance nitrifying sludge (HPNS) accession number NRRL-B-50298 and anammox bacterial sludge, Brocadia caroliniensis, accession number NRRL B-50286. The single-tank reactors were tested with digested swine wastewater. The reactors contained biofilm plastic carriers (30-40% volume/volume) that were fluidized by aeration. The process water temperature was ambient 23±2 degrees Celsius. Surprisingly, the two bacteria groups were able to associate quickly and effectively in the aerated single tanks providing a streamlined ammonia removal process. Microbial reverse transcription analyses indicated that bacteria in the digested swine influent had little effect on the bacterial community that was active in the single-tank. Results showed physiologically high activity of ammonia oxidizing bacteria (AOB) and anammox bacteria in the reactor. High rates of ammonia removal were obtained in a single tank (~ 1.0 kilogram nitrogen per cubic meter of reactor per day) with ammonia removal efficiency of 100%. Compared with traditional Nitrogen removal, the deammonification process reduced 56-57% of the aeration. The nitrogen was removed in a single-tank, further reducing equipment costs. Therefore, deammonification is a key technology for development of more economical and energy efficient biological ammonia removal systems.

Last Modified: 11/26/2015
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