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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC IMPROVEMENT OF BARLEY Title: Testing transgenic spring wheat and barley lines for reaction to Fusarium Head Blight: 2012 Field Nursery Report

Authors
item Koeritz, E.J. -
item Elakkad, A.M. -
item Dahleen, Lynn
item Skadsen, Ronald
item Abebe, T -
item Shah, J -
item Nalam, V.J. -
item Klossner, G -
item Turner, N -
item Di, R -
item Muehlbauer, G. J. -
item Li, X -
item Shin, S -
item Dill-Macky, R -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 29, 2012
Publication Date: December 4, 2012
Citation: Koeritz, E., Elakkad, A., Dahleen, L.S., Skadsen, R.W., Abebe, T., Shah, J., Nalam, V., Klossner, G., Turner, N., Di, R., Muehlbauer, G., Li, X., Shin, S., Dill-Macky, R. 2012. Testing transgenic spring wheat and barley lines for reaction to Fusarium Head Blight: 2012 Field Nursery Report. [Meeting Abstract.] 2012 National Fusarium Head Blight Forum. p 140.

Technical Abstract: The 2012 field screening nursery consisted of 42 wheat and 24 barley entries evaluated in side by side experiments. Entries within each species experiment were arranged in a randomized complete block design with four replications in a field located at UMore Park, Rosemount MN. Trial entries, and untransformed controls, were submitted by the University of North Texas (5+1 wheat), Rutgers University (7+1 wheat), the University of Minnesota (19+5 wheat), and the USDA (20+1 barley). Lines with known reactions to Fusarium head blight (FHB) were also included as checks. The wheat checks used were the moderately resistant Alsen, the susceptible cultivars Wheaton and Roblin, and a non-inoculated Wheaton check. The barley checks were the moderately resistant Quest and the susceptible cultivars, Robust and Stander. Individual plots were 2.4 m long single rows. The trial was planted on May 22, 2012. All plots, except a non-inoculated Wheaton check, were inoculated twice. The first inoculation, 6 July 2012, was applied at anthesis for wheat and at head emergence for barley. The second inoculation was applied three days after the initial inoculation (dai) for each plot. The inoculum was a composite of 30 F. graminearum isolates at a concentration of 100,000 macroconidia.ml-1 with Tween 20 (polysorbate) added at 2.5 ml.L-1 as a wetting agent. The inoculum was applied using a CO2-powered backpack sprayer fitted with a SS8003 TeeJet spray nozzle with an output of 10ml.sec-1 at a working pressure of 275 kPa. Mist-irrigation was applied from before the first inoculation on July 5 through July 25 to facilitate FHB development. FHB incidence and severity were assessed visually 19 d.a.i. for wheat and 14 d.a.i. for barley on 20 arbitrarily selected heads per plot. FHB incidence was determined by the percentage of spikes with visually symptomatic spikelets of the 20 heads observed. FHB severity was determined as the percentage symptomatic spikelets of the total of all spikelets observed. Plots were harvested at maturity on August 13 (barley) and August 22 (wheat). Fifty (barley) and 50 (wheat) heads where harvested from each plot, threshed and the seed cleaned manually. The wheat sub-samples were used to determine the percentage of visually scabby kernels (VSK) and then all samples (wheat and barley) were ground and submitted for deoxynivalenol (DON) analysis. The data indicated that resistance was expressed in some of the transformed lines. We would like to acknowledge Beheshteh Zargaran, Alencar Xavier, Rebecca Schneider, Jared Schuster and Vadym Matyash for their assistance in completion of the work.

Last Modified: 11/23/2014
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