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Research Project: MINING THE GENOME OF RHIPICEPHALUS MICROPLUS TO DEVELOP NOVEL CONTROL TECHNOLOGY AND VACCINES

Location: Tick and Biting Fly Research

Title: Transcriptome database derived from Australian population of the cattle tick, Rhipicephalus (Boophilus) microplus

Authors
item Heekin, Andrew
item Guerrero, Felix
item Lew-Tabor, A -
item Dowd, Scot
item Moolhuijzen, P -
item Bellgard, M -

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: May 7, 2012
Publication Date: N/A

Interpretive Summary: The Southern cattle tick, Rhipicephalus (Boophilus) microplus, vectors Babesia bovis and B. bigemina, which are the protozoans causing cattle fever, a disease that is responsible for significant production losses to cattle producers in much of Africa, Central and South America, and Australia. We initiated a study of the expressed genes of the synganglia, essentially the brain, of ticks collected from an Australian population in an attempt to identify tick genes which might be candidates for development of anti-tick or anti-Babesia control technologies. We applied a technique known as next-generation 454 pyrosequencing to dissected synganglia from the Australian ticks. Thus, we have produced a very large DNA sequence database consisting of gene coding regions from expressed genes in the synganglia of Australian ticks. The sets of sequences have been submitted and published under NCBI SRA Accession Number SRA052517.1.

Technical Abstract: The Southern cattle tick, Rhipicephalus (Boophilus) microplus, vectors Babesia bovis and B. bigemina, which are the protozoans causing cattle fever, a disease that is responsible for significant production losses to cattle producers in much of Africa, Central and South America, and Australia. We initiated a study of the transcriptome of synganglia from ticks collected from an Australian cattle tick population in an attempt to identify tick genes which might be candidates for development of anti-tick or anti-Babesia control technologies. We applied 454 pyrosequencing to dissected synganglia from the Australian ticks and have produced a very large DNA sequence database of this transcriptome. The sets of sequences have been submitted and published under NCBI SRA Accession Number SRA052517.1.

   

 
Project Team
Guerrero, Felix
Temeyer, Kevin
Miller, Robert
Li, Andrew
Perez De Leon, Adalberto - Beto
Olafson, Pia
Goolsby, John
Thomas, Donald
Osbrink, Weste
Showler, Allan
 
Publications
   Publications
 
Related National Programs
  Animal Health (103)
  Veterinary, Medical and Urban Entomology (104)
 
Related Projects
   INVESTIGATION OF ELECTROPORATION AS A MEANS OF TRANSFORMATION OF THE HORN FLY WITH GREEN FLUORESCENT PROTEIN
   SEQUENCING OF BAC ENDS FROM A RHIPICEPHALUS MICROPLUS BAC LIBRARY
   DEVELOPMENT OF A MALE-ONLY STRAIN OF THE NEW WORLD SCREWWORM, COCHLIOMYIA HOMINIVORAX (DIPTERA, CALLIPHORIDAE)
   SYSTEMS BIOLOGY INVESTIGATIONS OF ARTHROPOD-BORNE DISEASES TO CONTROL VECTORS OF PATHOGENS
   USE OF SYNERGIZED ACARICIDE FORMULATIONS FOR THE CONTROL OF RESISTANT POPULATIONS OF RHIPICEPHALUS MICROPLUS IN THE MEXICAN TROPICS
   INVESTIGATIONS OF NOVEL TARGET-SPECIFIC LIGANDS FOR ACETYLCHOLINESTERASES OF ARTHROPOD PESTS
   FUNCTIONAL CHARACTERIZATION AND TARGET VALIDATION OF TICK (BOOPHILUS MICROPLUS) G PROTEIN-COUPLED RECEPTORS
   CATTLE TICK GENOME SEQUENCING AND TRANSCRIPTOME ANALYSIS
   PRODUCTION AND EFFICACY TESTING OF BM86-TEXAS ANTI-TICK VACCINE FOR THE CATTLE FEVER TICK ERADICATION PROGRAM
   EVALUATION OF CANDIDATE ANTI-TICK VACCINE ANTIGENS
   EVALUATION OF IMMUNE RESPONSE OF WHITE-TAILED DEER FOLLOWING VACCINATION WITH ANTI-CATTLE TICK VACCINE CANDIDATE ANTIGENS
   EXPERIMENTAL BM86-BASED VACCINE FOR THE CATTLE FEVER TICK ERADICATION PROGRAM
   Evaluation of Immune Response of Canines Following Vaccination with Anti-Tick Vaccine Candidate Antigens
 
 
Last Modified: 05/22/2013
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