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United States Department of Agriculture

Agricultural Research Service

Research Project: CONTROL OF PATHOGENS IN STRAWBERRY AND VEGETABLE PRODUCTION SYSTEMS Title: Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire.

Authors
item Lévesque, C. André -
item Brouwer, Henk -
item Cano, Liliana -
item Hamilton, John -
item Holt, Carson -
item Huitema, Edgar -
item Raffaele, Sylvain -
item Robideau, Gregg -
item Win, Joe -
item Zerillo, Marcelo -
item Beakes, Gordon -
item Boore, Jeffrey -
item Busam, Dana -
item Dumas, Bernard -
item Ferriera, Steve -
item Fuerstenberg, Susan -
item Gachon, Claire M -
item Gaulin, Elodie -
item Govers, Francine -
item Grenville-Brigg, Laura -
item Horner, Neil -
item Hostetler, Jessica -
item Jiang, Rays H.Y. -
item Johnson, Justin -
item Krajaejun, Theerapong -
item Lin, Haining -
item Meijer, Harold J.G. -
item Moore, Barry -
item Morris, Paul -
item Phuntmart, Vipaporn -
item Puiu, Daniela -
item Shetty, Jyoti -
item Stajich, Jason -
item Tripathy, Sucheta -
item Wawra, Stephan -
item West, Pieter Van -
item Whitty, Brett -
item Coutinho, Pedro -
item Martin, Frank
item Thines, Marco -
item Thomas, Paul -
item Tyler, Brett -
item Vries, Ronald DE -
item Kamoun, Sophien -
item Yandell, Mark -
item Tisserat, Ned -
item Buell, C. Robin -

Submitted to: Genome Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 13, 2010
Publication Date: July 13, 2010
Repository URL: http://genomebiology.com/2010/11/7/R73
Citation: Lévesque, C., Brouwer, H., Cano, L., Hamilton, J.P., Holt, C., Huitema, E., Raffaele, S., Robideau, G.P., Win, J., Zerillo, M.M., Beakes, G.W., Boore, J.L., Busam, D., Dumas, B., Ferriera, S., Fuerstenberg, S.I., Gachon, C.M., Gaulin, E., Govers, F., Grenville-Brigg, L., Horner, N., Hostetler, J., Jiang, R., Johnson, J., Krajaejun, T., Lin, H., Meijer, H., Moore, B., Morris, P., Phuntmart, V., Puiu, D., Shetty, J., Stajich, J., Tripathy, S., Wawra, S., West, P., Whitty, B.R., Coutinho, P., Martin, F.N., Thines, M., Thomas, P.D., Tyler, B.M., Vries, R., Kamoun, S., Yandell, M., Tisserat, N., Buell, C. 2010. Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire.Genome Biology. 11:R73.

Interpretive Summary: This manuscript describes the sequencing of the nuclear genome of two isolates of the plant pathogen Pythium ultimum and its analysis.

Technical Abstract: The P. ultimum DAOM BR144 (=CBS 805.95 = ATCC200006) genome (42.8 Mb) encodes 15,290 genes, and has extensive sequence similarity and synteny with related Phytophthora spp., including the potato late blight pathogen Phytophthora infestans. Whole transcriptome sequencing revealed expression of 86 % of the genes, with detectable differential expression of suites of genes under abiotic and xenobiotic stress. Analysis of the predicted proteome revealed a large repertoire of proteins involved in plant pathogen interactions although surprisingly, the P. ultimum genome does not encode any classical RXLR effectors and only a few Crinkler genes, which are abundant in related phytopathogenic oomycetes. A lower number of enzymes involved in carbohydrate metabolism were present compared to Phytophthora species, with the notable absence of cutinases, suggesting a significant difference in virulence mechanisms between P. ultimum and more host specific oomycete species. Although we observed a high degree of orthology with Phytophthora genomes, there were novel features of the P. ultimum proteome including an expansion of genes involved in proteolysis and genes unique to Pythium. We identified a small gene family of cadherins, proteins involved in cell adhesion, the first report in a genome outside the metazoans. Access to the P. ultimum genome has revealed not only core pathogenic mechanisms within the oomycetes but also lineage specific genes associated with the alternative virulence and lifestyles found within the Pythiaceae compared to the Peronosporaceae.

Last Modified: 11/26/2014
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