ARS | Publication request: A method for selection of restriction enzymes for sdCAPS marker construction

Submitted to: Plant Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 20, 2011
Publication Date: September 25, 2011
Citation: Jeong, Y.S., Lee, S.Y., Choi, I.H., Lim, Y.P., Hur, Y.K., Staub, J.E., Chung, S.M. 2011. A method for selection of restriction enzymes for sdCAPS marker construction. Plant Breeding. 130:401-403.

Interpretive Summary: Biotechnology is widely used for the identification of plants. One technology that is important to plant identification for breeding and the application for legal plant variety protection is molecular marker technology. Molecular markers tag (associate with) DNA (the genetic information in a cell that deals with inheritance) in order to provide a precision identification of the DNA of one plant variety for comparisons with other varieties. There is a need to provide for more precise and simple techniques for plant identify characterization. Thus, experiments were designed to develop such a technology using DNA extracted from Chinese cabbage. A new technique was developed that is simpler and less expensive than those currently used. This technique has broad application to all major crop plants and thus can be used in the breeding and plant variety protection of U.S. varieties. Seed companies planning on patenting their intellectual property can use this technology. Application of this technology will allow for protection to consumers (U.S. producer) and thus will increase their competitiveness.

Technical Abstract: Development of PCR-based markers for SNP detection is prerequisite for various genetic analyses. The use of restriction enzymes following PCR amplification is a common and relatively low cost method for SNP detection. Simple and cost-effective methodologies for SNP marker development that would enhance the use of SNP-based technologies are desirable. As an alternative analytical method for selection of REs for recognition of SNP motifs for marker development is described herein that does not require computer-based selection of restriction enzymes. Given that only 12 restriction enzymes are required for the development of any SNP motif, the method described herein is relatively inexpensive and technically simple.