ARS | Publication request: Genetic management of broodstock populations with DNA markers in rainbow trout

Submitted to: World Congress of Genetics Applied in Livestock Production
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2010
Publication Date: August 1, 2010
Citation: Vallejo, R.L., Rexroad III, C.E., Liu, S., Palti, Y. 2010. Genetic management of broodstock populations with DNA markers in rainbow trout. World Congress of Genetics Applied in Livestock Production, Liepzig, Germany. CDROM.

Technical Abstract: DNA markers are very useful for aquaculture and fisheries broodstock management. They have been used for parentage assignment when spawning families share common environments, and to evaluate genetic parameters in broodstock populations. The selective breeding program at the National Center for Cool and Cold Water Aquaculture (NCCCWA) provides a unique resource for assessing accuracy of estimation of pair-wise relationship coefficients and population F-statistics with molecular data. We need this information as pair-wise relationships among the NCCCWA founder strains are unknown, and for assessing relatedness within/between hatchery stocks. The results indicate there is significant genetic differentiation (Fst=0.03-0.04) and population sub-structure (K=4) in the NCCCWA broodstock. Non-significant estimates of total inbreeding coefficient (Fit) and negative within-population inbreeding coefficient (Fis) indicate there is an excess of heterozygotes in the NCCCWA selective breeding population. Pair-wise relationship coefficients estimated with marker genotype data had low accuracy.