Submitted to: Biotechnology International Symposium Proceedings
Publication Type: Proceedings
Publication Acceptance Date: October 14, 2008
Publication Date: November 27, 2008
Citation: Wall, R.J. 2008. Gene Transfer in Livestock: Which Method should I use?. Biotechnology International Symposium Proceedings, p. 27-31, University of Santiago de Compostela, Spain. Interpretive Summary: The intentional introduction of recombinant DNA molecules into a living organism can be achieved in many ways. Viruses have been making a living by practicing gene transfer for millennia. Recently, man has gotten into the act. The paradigm employed is fairly straightforward. First, a way must be found to move genetic information across biological membrane barriers. Then, presumably, DNA repair mechanisms do the rest. The array of methods available to move DNA into the nucleus provides flexibility necessary to transfer genes into cells as physically diverse as sperm and eggs. The techniques differ widely in efficiency, degree of skill required and genetic manipulation possible. No approach can be considered better than all the others. The choice of gene transfer method must be based on the goals of the project and the resources on hand.
Technical Abstract: N/A