Skip to main content
ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #231116
Title: A real-time PCR assay for the detection of Pasteuria nishizawae in soil

Author
item ATIBALENTJA, NDEME - UNIVERSITY OF ILLINOIS
item BABADOOST, MOHAMMAD - UNIVERSITY OF ILLINOIS
item Noel, Gregory

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 3/11/2008
Publication Date: 7/26/2008
Citation: Atibalentja, N., Babadoost, M., Noel, G.R. 2008. A real-time PCR assay for the detection of Pasteuria nishizawae in soil [abstract]. American Phytopathological Society Annual Meeting. 98:S15.

Interpretive Summary:

Technical Abstract: Pasteuria nishizawae is a mycelial, endospore-forming, and obligately parasitic bacterium that has shown a great potential for the biological control of the soybean cyst nematode, Heterodera glycines, in both microplot and field studies. Other than China, Japan, and South Korea, P. nishizawae has been found naturally infecting H. glycines only in the United States (U.S.) at Urbana, Illinois. It is not known whether the bacterium occurs in other soybean fields in Illinois or elsewhere in the U.S., especially in fields where soil suppressiveness has been observed. Addressing this question requires development of a reliable assay to determine the presence, population density, and distribution of P. nishizawae in soybean fields. The current method that consists of counting the number of endospores attached to second-stage juveniles (J2) of H. glycines is not appropriate for that goal. We have developed a real-time quantitative PCR assay that is based on the 16S rRNA gene sequence of P. nishizawae. The assay has been successfully used to detect minute amounts (less than 1,000 copies) of the target from a crude DNA extract. This assay, when improved, will be very useful for conducting surveys to determine whether P. nishizawae occurs in soybean fields in the U.S.