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ARS Home » Northeast Area » Boston, Massachusetts » Jean Mayer Human Nutrition Research Center On Aging » Research » Publications at this Location » Publication #213870
Title: Postprandial Lipemia is Modified by the Presence of the APOB-516C/T Polymorphism in a Healthy Caucasian Population

Author
item PEREZ-MARTINEZ, PABLO - UNIV OF REINA SOFIA, ES
item PEREZ-JIMENEZ, FRANCISCO - UNIV OF REINA SOFIA, ES
item Ordovas, Jose
item MORENO, JUAN - UNIV OF REINA SOFIA, ES
item MARIN, CARMEN - UNIV OF REINA SOFIA, ES
item MORENO, RAFAEL - UNIV OF REINA SOFIA, ES
item JIMENEZ-GOMEZ, Y - UNIV OF REINA SOFIA, ES
item PANIAGUA, J - UNIV OF REINA SOFIA, ES
item LOPEZ-MIRANDA, JOSE - UNIV OF REINA SOFIA, ES

Submitted to: Lipids
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/2/2006
Publication Date: 3/1/2007
Citation: Perez-Martinez, P., Perez-Jimenez, F., Ordovas, J.M., Moreno, J.A., Marin, C., Moreno, R., Jimenez-Gomez, Y., Paniagua, J.A., Lopez-Miranda, J. 2007. Postprandial Lipemia is Modified by the Presence of the APOB-516C/T Polymorphism in a Healthy Caucasian Population. Lipids. 42(2):143-150.

Interpretive Summary: Coronary heart disease is modulated by genetic and environmental factors, namely, diet, physical activity, smoking and alcohol drinking. Many genes have been implicated in the risk for atherosclerosis and one of the best studied is the apolipoprotein B (APOB) gene coding for a protein that is of great importance for the metabolism of blood lipids. However, most studies carried out to establish the relation between blood lipids and heart disease have been carried out in the fasting state, which in our society is only present during 2-3 hours during day. Less is known about the relation between blood lipids in the non fasting state and heart disease. Previous studies suggest that the effect of the APOB variants on lipid parameters could be mediated through linkage to genes. We have investigated the relation between blood lipids following the consumption of a meal and the apoB-516C/T polymorphism in the APOB gene. Our data show that this mutation modulates the response to fat intake and this information can be used to provide more personalized dietary recommendations to prevent heart disease.

Technical Abstract: Apolipoprotein (apoB) plays a fundamental role in the transport and metabolism of plasma triacylglycerols (TAGs) and cholesterol. Several apoB polymorphic sites have been studied for their potential use as markers for coronary heart disease in the population. In view of the importance of apoB in postprandial metabolism, our objective was to determine whether the presence of the -516C/T polymorphism in the APOB gene promoter could influence postprandial lipoprotein metabolism in healthy subjects. Forty-seven volunteers who were homozygous for the E3 allele at the APOE gene were selected (30 homozygous for the common genotype (C/C) and 17 heterozygotes for the -516T allele (C/T). They were given a fat-rich meal containing 1 g fat and 7 mg cholesterol per kg body weight and vitamin A 60,000 IU/m(2) body surface. Fat accounted for 60% of calories, and protein and carbohydrates for 15 and 25% of energy, respectively. Blood samples were taken at time 0, every 1 h until 6 h, and every 2.5 h until 11 h. Total cholesterol and TAGs in plasma, and cholesterol, TAGs and retinyl palmitate in triacylglycerol-rich lipoproteins (large and small triacylglycerol-rich lipoproteins) were determined by ultracentrifugation. Individuals carrying the C/T genotype presented greater postprandial concentrations of TAGs in small triacylglycerol-rich lipoproteins than did carriers of the C/C genotype (P = 0.022). Moreover, C/T individuals presented higher concentrations of plasma TAGs during the postprandial period than did C/C subjects (P = 0.039). No other statistically significant genotype-related differences for other parameters were observed. These results suggest that the presence of the genotype C/T is associated with a higher postprandial response. Thus, the allele variability in the -516C/T polymorphism in the APOB gene promoter may partly explain the interindividual differences in postprandial lipemic response in healthy subjects.