|Galpin, Lauren - WASH UNIV SCH MEDICINE|
|Fleming, Katherine - WASH UNIV SCH MEDICINE|
|Ou, Ching-Nan - BAYLOR COLLEGE MED|
|Ashorn, Per - TAMPERE INIV HOSPITAL|
Submitted to: American Journal of Clinical Nutrition
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 21, 2005
Publication Date: November 1, 2005
Citation: Galpin, L., Manary, M.J., Fleming, K., Ou, C., Ashorn, P., Shulman, R.J. 2005. Effect of Lactobacillus GG on intestinal integrity in Malawian children at risk of tropical enteropathy. American Journal of Clinical Nutrition. 82(11):1040-1045. Interpretive Summary: Children in tropical third world countries are at risk for developing intestinal damage. Probiotics ("good bacteria") have been shown in some circumstances to protect against intestinal infections and other intestinal ailments. This study tested the ability of a well-known probiotic that has been shown to be effective in childhood diarrhea caused by the virus Rotavirus to protect against tropical intestinal damage in children in Malawi. Children received either the probiotic Lactobacillus GG or placebo. The results indicated that the probiotic in this situation did not protect against intestinal damage in this population of children.
Technical Abstract: Background: Tropical enteropathy is an asymptomatic villous atrophy of the small bowel that is prevalent in the developing world and is associated with altered intestinal function and integrity. The histology of tropical enteropathy resembles that seen in small-bowel bacterial overgrowth. Objective: This study tested the hypothesis that treatment of 3- to 5-y-old Malawian children with the probiotic Lactobacillus GG would improve their intestinal function and integrity. Design: Clinically healthy children (n = 164) were enrolled in a placebo-controlled, randomized, double-blind trial. Intestinal function and integrity were measured by using the site-specific sugar-absorption test before and after 30 d of treatment with Lactobacillus GG or placebo. The primary outcomes were the ratios of urinary lactulose to mannitol (L:M) and of urinary sucrose to lactulose (S:L) excretion. Results: Of the 161 children who completed the study, 119 (73%) had tropical enteropathy on enrollment (L:M > 0.10). Children receiving Lactobacillus GG did not differ significantly from the placebo group in the excretion (in % of dose administered) of mannitol (mean +/- SD: 8.9 +/- 4.4 and 8.9 +/- 3.9, respectively), lactulose (0.31 +/- 0.20 and 0.33 +/- 0.23, respectively), or sucrose (0.078 +/- 0.058 and 0.082 +/- 0.075, respectively). L:M and S:L also did not differ significantly between the Lactobacillus and placebo groups (0.19 +/- 0.13 and 0.20 +/- 0.12, respectively, for L:M; 0.58 +/- 0.46 and 0.65 +/- 0.57,respectively, for S:L). Conclusion: Administration of Lactobacillus GG for 30 d had no effect on the intestinal integrity of 3- to 5-y-old Malawian children.