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United States Department of Agriculture

Agricultural Research Service

Title: Functional Genomic Analysis of the Auxin/indole-3-Acetic Acid Gene Family Members in Arabidopsis Thaliana

Authors
item Overvoorde, Paul - ARS-UCB PLNT GENE EXP CTR
item Okushima, Yoko - ARS-UCB PLNT GENE EXP CTR
item Alonso, Jose - SALK INST, LA JOLLA CA
item Chan, April - ARS-UCB PLNT GENE EXP CTR
item Chang, Charlie - ARS-UCB PLNT GENE EXP CTR
item Ecker, Joseph - SALK INST, LA JOLLA CA
item Hughes, Beth - ARS-UCB PLNT GENE EXP CTR
item Liu, Amy - ARS-UCB PLNT GENE EXP CTR
item Onodera, Courtney - ARS-UCB PLNT GENE EXP CTR
item Quach, Hong - ARS-UCB PLNT GENE EXP CTR
item Smith, Alison - ARS-UCB PLNT GENE EXP CTR
item Yu, Guixia - ARS-UCB PLNT GENE EXP CTR
item Theologis, Athanasios

Submitted to: The Plant Cell
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 3, 2005
Publication Date: November 11, 2005
Repository URL: http://www.plantcell.org/cgi/reprint/17/12/3282
Citation: Overvoorde, P.J., Okushima, Y., Alonso, J.M., Chan, A., Chang, C., Ecker, J.R., Hughes, B., Liu, A., Onodera, C., Quach, H., Smith, A., Yu, G., Theologis, A. 2005. Functional Genomic Analysis of the AUXIN/INDOLE-3-ACETICc ACID Gene Family Members in Arabidopsis thaliana. The Plant Cell. 17:3282-3300.

Interpretive Summary: Auxin regulates various aspects of plant growth and development. The AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) genes regulate auxin-mediated gene expression by interacting with members of the AUXIN RESPONSE FACTOR protein family. Aux/IAA function is poorly understood; herein, we report the identification and characterization of insertion mutants in 12 of the 29 Aux/IAA family members. The data suggest extensive functional redundancy among Aux/IAA gene family members and that enhanced stability of the AXR3/IAA17 protein severely alters the molecular phenotype, resulting in developmental defects.

Technical Abstract: Auxin regulates various aspects of plant growth and development. The AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) genes encode short-lived transcriptional repressors that are targeted by the TRANSPORT INHIBITOR RESPONSE1/AUXIN RECEPTOR F-BOX proteins. The Aux/IAA proteins regulate auxin-mediated gene expression by interacting with members of the AUXIN RESPONSE FACTOR protein family. Aux/IAA function is poorly understood; herein, we report the identification and characterization of insertion mutants in 12 of the 29 Aux/IAA family members. The mutants show no visible developmental defects compared with the wild type. Double or triple mutants of closely related Aux/IAA genes, such as iaa8-1 iaa9-1 or iaa5-1 iaa6-1 iaa19-1, also exhibit wild-type phenotypes. Global gene expression analysis reveals that the molecular phenotypes of auxin-treated and untreated light-grown seedlings are unaffected in the iaa17-6 and iaa5-1 iaa6-1 iaa19-1 mutants. By contrast, similar analysis with the gain-of-function axr3-1/iaa17-1 mutant seedlings reveals dramatic changes in basal and auxin-induced gene expression compared with the wild type. Expression of several type-A ARABIDOPSIS RESPONSE REGULATOR genes and a number of genes involved in cell wall biosynthesis and degradation is repressed in axr3-1/iaa17-1. The data suggest extensive functional redundancy among Aux/IAA gene family members and that enhanced stability of the AXR3/IAA17 protein severely alters the molecular phenotype, resulting in developmental defects.

Last Modified: 9/23/2014
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