Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Isolation and Characterization of a Truncated Member (Mild-1c) of the Miniature Inverted-Repeat Transposable Element Family Mild-1 from the Colorado Potato Beetle, Leptinotarsa Decemlineata.

Author
item YOCUM, GEORGE

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: May 18, 2005
Publication Date: May 23, 2005
Citation: Yocum, G.D. 2005. Isolation and characterization of a truncated member (mild-1c) of the miniature inverted-repeat transposable element family mild-1 from the colorado potato beetle, Leptinotarsa decemlineata. Genbank. Accession number: DQ011147.

Technical Abstract: Using PCR and genomic walking, the sequence of the DAT-3 gene and its flanking regions were determined for a final construct of 3984 bp in length. The gene of DAT-3 is composed of two exons 210 and 148 in bp length separated by a 1107 bp intron. Southern blot experiments demonstrated that there is only a single copy of DAT-3 in the Colorado potato beetle genome. Determining the genomic structure of DAT-3 led to the isolation of three novel families of miniature inverted-repeat transposable elements (Mild-1, -2 and 3). Mild-1a is inserted within the first intron of diapause protein-1. Mild-1a is 284 bp in length, has a 14 bp target site duplication, three sets of subterminal inverted-repeats, is TA rich and can form a hairpin structure. The second element, Mild-2a, is inserted within the 3' terminus of Mild-1a. Mild-2 is 29 bp in length with a 3 bp target site duplication and one set of subterminal inverted-repeats. Mild-3a shares 60% identity with Mild-1a, is 251 bp in length, has a 8 bp target site duplication and has one set of subterminal inverted repeats that form a hairpin structure. The target site duplications of Mild-1 and -3 families share a common conserved core of AATTT. Three truncated members of the Mild-1 family and one truncated member of the Mild-2 family were isolated and provide evidence for a past history of MITE transposition. Accession number: DQ011147

Last Modified: 9/10/2014
Footer Content Back to Top of Page