Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: March 19, 2005
Publication Date: March 19, 2005
Citation: Palti, Y., Fincham, M.R., Rodriguez, M.F., Gahr, S.A., Rexroad III, C.E., Hansen, 2005. J. Genomic sequencing of a rainbow trout major histocompatibility region that harbors class ii and class iii genes. Plant and Animal Genome XIII Abstracts P606, pg 222. Technical Abstract: Major histocompatibility complex (MHC) class I and II molecules are surface glycoproteins that present self and foreign peptides to the cellular arm (T-cells) of the immune system through a process known as antigen presentation. The human class III region contains a mix of genes that are structurally and functionally unrelated and is physically located between the Class I and class II regions. We have previously shown that the rainbow trout genome contains at least 4 unlinked regions of major histocompatibility (MH) genes. In the current study, we developed BAC contigs for a region that harbors several class II and III genes and spans approximately 300 kbp. We have sequenced and annotated 3 of the BACs since one of our primary goals is to provide a clear definition of MH haplotypes for rainbow trout. The region contain ORFs with high sequence homology to class II genes such as ABCB2 (aka TAP1), BRD2 (aka RING3) and RAB2 (aka RGL2), class III genes such as PBX2 and NOTCH4 and a few novel genes with motifs that are found in other immune response genes. The genomic organization and gene content will be compared to MH regions in other species and the significance of the findings for fish health will be addressed.