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United States Department of Agriculture

Agricultural Research Service

Title: DEVELOPMENT AND MAPPING OF WHEAT MICROSATELLITE MARKERS

Authors
item Song, Qijian - CHINA
item Shi, J - MICHIGAN STATE UNIVERSITY
item Singh, S - KANSAS STATE UNIVERSITY
item FICKUS, EDWARD
item Fernalld, Raymond
item Gill, B - KANSAS STATE UNIVERSITY
item CREGAN, PERRY
item Ward, R - MICHIGAN STATE

Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: December 15, 2001
Publication Date: N/A

Technical Abstract: Microsatellite DNA markers have been demonstrated to be relatively informative in hexaploid wheat. The objectives of this research were to develop new wheat microsatellite markers mostly based on (ATT) motifs and to place them on the ITMI genetic map and by physical mapping with nullisomic-tetrasomic stocks. A total of 410 markers were developed from random genomic libraries, and among them, 250 markers were polymorphic among Chinese Spring, Opata and M6. One hundred and forty-four of these markers were polymorphic in the ITMI mapping population and were positioned on the ITMI map, seventy three were positioned using nulli- tetrasomic lines of Chinese Spring. A total of 70, 82 and 65 markers were mapped on A, B, and D genomes respectively. New mocrosatellites were integrated into a framework map consisting of previously published RFLP and microsatellite markers. The previous linkage maps were extended by a total of 67.2 cM. A total of 55, 23 and 40 of the new markers were positioned in gaps larger than 30, 20 and 10cM, respectively.

Last Modified: 8/27/2014
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