|Mahama, A - IOWA STATE UNIVERSITY|
Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 10, 2001
Publication Date: November 3, 2001
Citation: MAHAMA, A.A., PALMER, R.G. GENETIC LINKAGE BETWEEN SOYBEAN MUTANTS AND TRANSLOCATION BREAKPOINTS. AGRONOMY ABSTRACTS. 2001. CO1-MAHAMA150941-POSTER. Technical Abstract: Reciprocal translocations are important for locating genes to linkage groups (LGs). Identifying the chromosomes involved in translocations is necessary for the isolation of tester sets. Our objectives were to: 1) test whether classical LGs (CLGs) 6 and 8 of soybean are the same or different; 2) determine the location of the breakpoints in three translocations in relation to mutants of CLGs 6 (Df2, Y11) and 8 (Adh1, Ms1, Ms6, St5, W1, Y23,); and 3) verify the homology of translocated segments. The KS172-11-3, KS175-7-3, and Clark T/T, translocations were crossed to the same genetic marker types. F2 seed was increased at the University of Puerto Rico/Iowa State University soybean nursery, near Isabela, PR. Data for the different characters were collected from F2 and F2:3 families grown at the Bruner Farm near Ames, IA. Recombination values revealed that all translocations were independent of T of CLG 1, and Y11, St5 and Adh1 of CLGs 6 and 8 but all three were linked to Df2, Ms1, W1, Y23; and Clark T/T was linked to Ms6. Our data suggest that for all three translocations, the breakpoint is between Df2 and Ms1, that CLGs 6 and 8 are the same LG, and that the three translocations have a common chromosome. This information will be useful in assignment of CLGs, in the isolation of a tester set of translocations, and will enhance genetic linkage mapping.