Submitted to: Scientia Agricola
Publication Type: Abstract Only
Publication Acceptance Date: August 1, 1998
Publication Date: N/A
Technical Abstract: Application of DNA technology in seed research has progressed rapidly during the last ten years, especially in the area of cultivar identification. The Restricted Fragment Length Polymorphism (RFLP) technique was first developed in 1980. Its application to cultivar identification, however, was not reported until almost a decade later (Nagamine et al, 1989). More recently, molecular marker techniques based on the Polymerase Chain Reaction (PCR) have become increasingly popular for fingerprinting and cultivar identification. Examples of PCR-based techniques include the Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP) and Simple Sequence Repeats (SSR) techniques. Future DNA marker technology will rely more on automation and is moving toward the recently- invented Single Nucleotide Polymorphism (SNP) technique using DNA chip technology. DNA markers will be increasingly utilized in molecular breeding to accelerate the backcrossing process during cultivar development. The genetic transformation of many crop and ornamental species is also becoming more routine. The effects of the transgenes on seed vigor and seed storability remain unknown. Because transgenic seeds are more costly than conventional seeds, there has been a great deal of interest in identifying genes or DNA markers associated with seed vigor and seed storability. However the progress has been slow. Efforts are underway for mapping the Quantitative Trait Loci (QTL) for seed storability. Once the DNA markers or genes associated with seed storability are found, they can be used in molecular breeding or genetic transformation to improve seed storability of economically important seeds.