Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: New Characters for Nematodes - Specific Gravity and Comparative Biocontrol Bacteria Sensitivity

Author
item Carta, Lynn

Submitted to: BARC Poster Day
Publication Type: Abstract Only
Publication Acceptance Date: April 8, 1999
Publication Date: N/A

Technical Abstract: An important aspect of nematode damage assessment is distinguishing species after extraction from soil. The cleanest, most efficient separation procedure employs a 1M sugar solution which extracts all nematodes in an often dehydrated and distorted condition, reducing their shelf-life. This "one-size-fits-all" solution has obscured real density differences in different groups of nematodes. This study outlines an approach to determine the density profile of any nematode group, (or other particle) including the mean and standard deviation of its population distribution. The results indicate that measurable differences exist among families, species and even stages of different nematodes. This knowledge can be used to design gentler, cleaner separation protocols. Our lab is also providing the first systematic data on the ability of different taxonomic groups of nematodes to grow and be attracted to biocontrol bacteria to predict and improve effectiveness. Nematodes most closely related to plant parasites that tolerate dry soil are better able to survive bacterial toxins than more distantly related taxonomic groups. This information will be useful when we compare the codes for toxin sensitive genes in different nematodes for developing new diagnostic characters for plant parasitic nematodes. (See FILLING IN THE GAPS OF THE LESION NEMATODE FAMILY TREE WITH OLD AND NEW MOLECULAR CHARACTERS Andrea M. Skantar and Lynn Carta)

Last Modified: 7/30/2014