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United States Department of Agriculture

Agricultural Research Service

Title: Analytical Supercritical Fluid Extraction (Sfe) for the Determination of Oil and Fat Content: Dependence on the Sample Matrix

item King, Jerry
item Eller, Fred
item Taylor, Scott

Submitted to: Fine Particle Society
Publication Type: Abstract Only
Publication Acceptance Date: April 3, 1998
Publication Date: N/A

Technical Abstract: Analytical supercritical fluid extraction using carbon dioxide(SC-CO2) as the extraction fluid has shown considerable promise as an alternative technique for the determination of lipid moieties in a wide variety of matrices. Both gravimetry and the more specific Nutritional Labeling & Education Act (NLEA) gas chromatographic method have been used after extraction for the nonspecific and specific determination of lipid species However, results from the SFE technique or any other extraction method can be dependent on preextraction sample preparation treatment, such as acidic or enzymatic hydrolysis. SFE can be used for determining the fat oil content of sample matrices such as meat products, oilseeds, and cereal based bakery products. In general our results indicate that gravimetry and gas chromatography (GC) give similar results, but gravimetric determination slightly overestimates lipid content. This has also been confirmed on National Institute of Standards and Technology (NIST) standard reference materials. For matrices having high carbohydrate content, the SFE results are equivalent using both GC and gravimetric determination on samples with high lipid content, when using ethanol as a cosolvent. Discrepencies arise, however, between the two determinative methods when the lipid content of the high carbohydrate matrix is low.

Last Modified: 4/22/2015
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