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United States Department of Agriculture

Agricultural Research Service

Title: Molecular Markers Linked to Quantitative Trait Loci for Anthracnose Resistance in Tomato

Authors
item Stommel, John
item Zhang, Yiping

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 1998
Publication Date: N/A

Technical Abstract: Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers linked to quantitative trait loci (QTL) involved in tomato anthracnose resistance were identified in an F2 population of tomato (Lycopersicon esculentum) segregating for anthracnose resistance. The F2 population was developed from the cross of an unadapted and small-fruited, but highly anthracnose resistant L. esculentum accession and an adapted, but anthracnose susceptible processing type tomato. Resistance to anthracnose caused by the fungal pathogen Colletotrichum coccodes is estimated to be controlled by at least three genes or chromosomal regions in this cross. One-thousand RAPD random primers and 64 AFLP primer pairs were screened for polymorphisms between the parental lines. Primers or primer pairs which differentiated the anthracnose resistant and susceptible parents were utilized to screen the F2 population for detection of QTL. Using single-factor analysis of variance, a number of markers, including six unmapped RAPD markers were identified which were significantly associated with resistance. Mapping of marker loci and their potential use in marker assisted breeding will be discussed.

Last Modified: 12/21/2014