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United States Department of Agriculture

Agricultural Research Service

Title: Hydrodynamic Chromatography and Laser Light Scattering Measurements of High Molecular Weight Waxy Maize Amylopectins

Authors
item Klavons, Jerome
item Dintzis, Frederick
item Millard, Merle

Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 4, 1996
Publication Date: N/A

Technical Abstract: The application of conventional Size Exclusion Chromatography to the fractionation of branched polysaccharides has, at present, an upper molecular weight limit of approximately twenty million. Waxy maize amylopectins with weight average molecular weights (Mw) up to approximately 100 million daltons, as measured by multi-angle laser light scattering (MALLS), have been fractionated using a commercial solid bead hydrodynamic column with 90% DMSO-H2O (w/w) as the mobile phase. Our work with this chromatographic system, consisting of a hydrodynamic column, a commercial dual angle laser light scattering detector with associated software, and a differential refractive index detector, indicates that still higher molecular weights could be fractionated by this type of column. Current developments and problems in examining these extremely high molecular weight, highly branched, polysaccharides will be discussed.

Last Modified: 4/19/2014
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