Technical Abstract: A cotton genomic library was screened using a fiber-specific cDNA (GH3) encoding a lipid transfer protein (LTP). One genomic clone (1.7 kb DNA insert) containing the Ltp gene (Ltp6) was sequenced and characterized. The Ltp6 contains an open reading frame of 360 bp, which is interrupted by a single intron (136 bp) located in the region corresonding to the C-terminal of the protein. The derived amino acid sequence of LTP6 is 64% homologous to that of GH3. Like the GH3 gene, the Ltp6 is specifically expressed in fiber cells in a temporal manner. However, its expression level is lower than that of GH3. The 5' flanking region of Ltp6 was cloned into a GUS (B-glucuronidase) gene fusion plasmid pBI121 and was shown to contain promoter activity after introduction into tobacco NT1 cells using a biolistic transformation technique.