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United States Department of Agriculture

Agricultural Research Service

Title: Cloning and Characterization of a Cotton Lipid Transfer Protein Gene Specifically Expressed in Fiber Cells

Authors
item Ma, Din - MISSISSIPPI STATE UNIV
item Liu, Hsi - MISSISSIPPI STATE UNIV
item Tan, Hingpoh - MISSISSIPPI STATE UNIV
item Creech, Roy - MISSISSIPPI STATE UNIV
item Jenkins, Johnie
item Chang, Yung - MISSISSIPPI STATE UNIV

Submitted to: Biochimica et Biophysica Acta
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 4, 1996
Publication Date: N/A

Interpretive Summary: Fiber development in cotton is regulated by several genes with specific function which operate in a spatial and temporal fashion during fiber development. A cotton genomic library was screened using a fiber-specific cDNA (GH3) encoding a lipid transfer protein (LTP). One genomic clone (1.7 kb DNA insert) containing the Ltp gene (Ltp6) was sequenced and characterized. This gene is expressed in fiber cells and may be involved in the synthesis of cutin during fiber development. The 5' flanking region of Ltp6 contains the promoter function. Synthesis of fiber specific genes and promoters should further the understanding of the physiology and genetics of fiber development in cotton.

Technical Abstract: A cotton genomic library was screened using a fiber-specific cDNA (GH3) encoding a lipid transfer protein (LTP). One genomic clone (1.7 kb DNA insert) containing the Ltp gene (Ltp6) was sequenced and characterized. The Ltp6 contains an open reading frame of 360 bp, which is interrupted by a single intron (136 bp) located in the region corresonding to the C-terminal of the protein. The derived amino acid sequence of LTP6 is 64% homologous to that of GH3. Like the GH3 gene, the Ltp6 is specifically expressed in fiber cells in a temporal manner. However, its expression level is lower than that of GH3. The 5' flanking region of Ltp6 was cloned into a GUS (B-glucuronidase) gene fusion plasmid pBI121 and was shown to contain promoter activity after introduction into tobacco NT1 cells using a biolistic transformation technique.

Last Modified: 12/20/2014
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