ARS | Publication request: MONILIFORMIN, FUMONISIN B1 AND TWO BETA-D-FRUCTOSE ANALOGS PRODUCED BY FUSARIUM SPP.: BIOLOGICAL ACTIVITIES TOWARDS LEMNA MINOR AND PRIMARY CHICK HEPATOCYTES

Submitted to: Phytochemical Society of North America Meeting and Newsletter
Publication Type: Abstract Only
Publication Acceptance Date: August 14, 1996
Publication Date: N/A

Technical Abstract: Metabolites isolated from Fusarium spp. by means of duckweed (Lemna minor L.) bioassay-directed fractionation are: 2,5-anhydro-D- mannitol (ADM), 2,5-anhyro-D-sorbitol (ADS) and moniliformin (MON) from F. solani; fumonisin B1 (FB1) from F. moniliforme; and MON and FB1 from F. proliferatum. ADM has been reported as a regulator of carbohydrate metabolism in hepatocytes isolated from diabetic, fasted rats. Since the above compounds inhibited duckweed [IC50 (ug/mL) MON (100), ADM (55), ADS (69), FB1 (0.7)], all were tested for their abilities to inhibit gluconeogenesis (GLN) in hepatocytes isolated from 10-day chick embryo. ADM, ADS and MON inhibited GLN from the substrate lactate plus pyruvate at IC50 of 6 mM, 12 mM and .2 mM, respectively. Under these same conditions, FB1 did not inhibit GLN at 1 mM. This is the first observation of MON and the fructose analogs ADM and ADS as carbohydrate metabolism regulators in chick hepatocytes. Since the Fusarium strains were isolated either from toxic chicken feeds or from roots of soybean plants exhibiting necrosis, production of carbohydrate metabolism regulators may explain the observed toxicities.