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United States Department of Agriculture

Agricultural Research Service

Title: Development of a Monoclonal Antibody-Based Elisa for the Sulfonamide Antibiotic Sulfadimethoxine: Application for the Analysis of Incurred Residue Tissues

item Muldoon, Mark
item Buckley, Sandra
item Rocco, Richard - IDETEK, INC
item Deshpande, Sudhir - IDETEK, INC
item Stanker, Larry

Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: May 2, 1996
Publication Date: N/A

Technical Abstract: The sulfonamide class of antibiotics are widely used in animal husbandry to treat bacterial and protozoan infections. Due to their extensive use, residues have been reported in tissues from a variety of food animals. Currently in the U.S., the tolerance level for sulfonamides in animal-based food products is 0.1 ppm. Analytical methods for sulfonamides include bioassays, TLC, HPLC, and immunoassays. The majority of immunoassays have used polyclonal antibodies. In this study, we report the development of a panel of murine monoclonal antibodies (mAbs) against sulfadimethoxine. Using sulfadimethoxine as the immunizing hapten, the mAbs exhibited a variety of crossreactivity patterns against the agriculturally-important sulfonamides sulfadimethoxine, sulfathiazole, and sulfamethazine using a heterologous, indirect enzyme-linked immunosorbent assay (ELISA) format. The most sensitive and specific ELISA gave an IC50 for sulfadimethoxine of 1.5 ppb and a limit of detection of approximately 0.2 ppb. The ELISA was used for the analysis of porcine tissues obtained from an incurred residue study. The results were validated by an HPLC method and will be further discussed. The ELISA method should save time and expense for residue monitoring of animal tissues.

Last Modified: 4/17/2015
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