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United States Department of Agriculture

Agricultural Research Service

Title: Development of a Monoclonal Antibody-Based Immunoassay for the Sulfonamide Antibiotic Sulfapyridine

item Muldoon, Mark
item Font, Ivan
item Young, Colin
item Stanker, Larry

Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: December 15, 1996
Publication Date: N/A

Technical Abstract: The sulfonamide class of antibiotics are widely used in veterinary medicine. Due to their extensive use, it is necessary to monitor for residues in various animal-based foods. Current analytical methods for the detection of sulfonamide antibiotics involve solvent extraction and cleanup followed by chromatography. Immunoassays have been developed for some sulfonamide antibiotics but few have utilized monoclonal antibodies. We have developed a sensitive monoclonal antibody-based immunoassay for sulfaphyidine. An aminobenzoic acid sulfonamide hapten-protein conjugate was synthesized and used to immunize five mice. Only one mouse tested positive for antibodies which could be used to detect relevant sulfonamide drugs. A rare antibody-secreting hybridoma was isolated that was highly sensitive for sulfapyridine (IC50=25 ppb) and showed cross reactivity toward other sulfonamides. Results from molecular modeling studies of the hapten and various sulfonamide drugs suggested that antibody recognition was dependent on N**1 aromatic ring substitution and will be further discussed.

Last Modified: 4/21/2015