Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Short Communication: Construction and Characterization of a Large Insert Bovine Yac Library with 5-Fold Genomic Coverage

Authors
item Smith, Timothy
item Alexander, Leeson
item Sonstegard, Tad
item Yoo, Jakyoung - UNIV. CA., BERKELEY
item Beattie, Craig
item Broom, M - UNIV. OTAGO, NEW ZEALAND

Submitted to: Mammalian Genome
Publication Type: Abstract Only
Publication Acceptance Date: December 19, 1995
Publication Date: N/A

Technical Abstract: Genetic linkage maps of the bovine genome have recently become available and are now being used to identify markers associated with traits of economic importance (ETL). Once mapped with available markers, fine mapping of these loci will be greatly facilitated by the availability of large insert libraries from which additional markers can be rapidly obtained. In the human and mouse systems, yeast artificial chromosome (YAC) vectors have been used with great success for the construction of contigs in the vicinity of target genes. Contig construction has depended on the availability of multiple YAC libraries that provide depth of coverage to minimize the impact of chimeric and deleted clones inherent in these libraries. A single bovine genomic YAC library has been reported but is commercially owned and not universally available. We report the construction of a bovine YAC library with approximately 5-fold coverage of the genome and a low rate of chimerism, to provide a public resource for positional cloning.

Last Modified: 4/16/2014