ARS | Publication request: DETECTION OF BARLEY YELLOW DWARF VIRUS-PAV-IL USING DIFFERENT TECHNIQUES

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: August 7, 1995
Publication Date: N/A

Technical Abstract: The sensitivity of an improved nucleic acid hybridization technique, using chromogenic and chemiluminescent substrates, was compared to PCR, double antibody sandwich and triple antibody sandwich ELISA for the detection of BYDV-PAV-IL in extracts of infected leaves and in purified virus preparations. The sensitivities of DAS and TAS-ELISA were similar, detecting 1 ng of purified PAV and the equivalent of 28 ng of infected tissue. Nucleic acid hybridization techniques using both substrates also detected 1 ng of purified PAV and virus in the equivalent of 25 ng of infected plant tissue. PCR was the most sensitive of the three techniques and detected 0.1 pg of RNA extracted form purified virus and the RNA from the equivalent of 0.5 pg of infected leaf tissue.