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United States Department of Agriculture

Agricultural Research Service

Title: Analyses of Mab Reactive with Porcine Cd6

Authors
item Saalmuller A, - TUBINGEN, GERMANY
item Aasted B, - ROYAL VET UNIV, DENMARK
item Canals A, - INIA, MADRID SPAIN
item Dominguez J, - INIA, MADRID SPAIN
item Goldman Theresa,
item Lunney, Joan
item Maurer S, - TUBINGEN, GERMANY
item Pauly T, - TUBINGEN, GERMANY
item Pescovitz M D, - IN UNIV, INDIANA
item Pospisil R, - PRAGUE, CZECH REPUBLIC

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 1, 1994
Publication Date: N/A

Interpretive Summary: This manuscript summarizes the results of part of the second round of analyses performed by scientists participating in the First International Swine CD Workshop. This workshop was set up to compare and standardize the reactivities of monoclonal antibodies (mAb) that recognize pig leukocyte cell surface markers to assign them specific cluster of differentiation numbers (CD#). For the second round analyses, the reactivities of 54 mAbs on T cell subsets or lines were analyzed by flow cytometry a nd the data subjected to statistical clustering to identify groups of mAbs that reacted similarly with specific pig T cells. Based on FCM analyses, two mAb recognizing the porcine CD6 analogue could be identified. The observed molecular mass of 110 kDaR of the antigen precipitated by mAb a38b2 confirmed this classification. Clustered mAbs were examined in more detail to determine whether they bound to the same area, or epitope, of the CD antigen in the later stages of the 2nd round of workshop analyses. Becaus of missing analyses of the molecular mass of the PG90A-antigen, and epitope blocking studies employing mAb a38b2, the membership of mAb PG90A to the CD6 cluster is not absolutely sure and therefore this mAb is allocated to the wCD 6 subcluster. These assignments will enable researchers to compare their scientific data using anti-pig T cell CD6 mAb.

Technical Abstract: Based on FCM analyses, two mAb recognizing the porcine CD6 analogue could be identified. The observed molecular mass of 110kDaR of the antigen precipitated by mAb a38b2 (#120), confirmed this classification. Because of missing analyses of the molecular mass of the PG90A-antigen and epitope blocking studies employing mAb a38b2, the membership of mAb PG90A (#055) to othe CD6 cluster is not absolutely sure and therefore this mAb is allocated to the wCD6 subcluster.

Last Modified: 12/21/2014
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