Author
Lin, Hong | |
PIETERSEN, GERHARD - University Of Pretoria | |
HAN, CLIFF - Los Alamos National Research Laboratory | |
READ, D. ALAN - University Of Pretoria | |
LOU, BINGHAI - Guangxi Citrus Research Institute | |
GUPTA, GOUTAM - Los Alamos National Research Laboratory | |
Civerolo, Edwin |
Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 6/15/2015 Publication Date: 7/16/2015 Citation: Lin, H., Pietersen, G., Han, C., Read, D., Lou, B., Gupta, G., Civerolo, E.L. 2015. Complete genome sequence of ‘Candidatus Liberibacter africanus’. Genome Announcements. 3(4):300733-15. doi: 10.1128/genomeA.00733-15.. Interpretive Summary: Huanglongbing is a devastating disease of citrus caused by the bacterium, “Candidatus Liberibacter spp”. The complete genome sequence of ‘Candidatus Liberibacter africanus’ (Laf) strain ptsapsy was obtained by high through-put next generation sequencing technology. The Laf strain genome possesses a single circular chromosome of 1,192,232 nucleotides. A total 1,141 genes were predicted based on computational analysis. The complete genome sequence of Laf provides new information for identification of critical genes and facilitates development of effective strategies to control this economically important disease. Technical Abstract: The complete genome sequence of ‘Candidatus Liberibacter africanus’ (Laf), strain ptsapsy, was obtained by an Illumina HiSeq 2000. The Laf genome comprises 1,192,232 nucleotides, 34.5% GC content, 1,141 predicted coding sequences, 44 tRNAs, 3 complete copies of ribosomal RNA genes (16S, 23S and 5S) and 368 hypothetical genes. In addition, two tandem aligned prophage segments were identified in the Laf genome. The average GC content in this prophage region is ~42.1%, deviating significantly from the core genome. This Laf genome sequence facilitates comparative genome analysis and advances in understanding of pathogenicity of citrus huanglongbing disease. |