Author
IGARASHI, YASUHIRO - Toyama University | |
GOHDA, FUMIYA - Toyama University | |
KADOSHIMA, TAITO - Toyama University | |
FUKUDA, TAKAO - Toyama University | |
HANAFUSA, TOMOAKI - Toyama University | |
SHOJIMA, AKANE - Kyushu University | |
NAKAYAMA, JIRO - Kyushu University | |
BILLS, GERALD - University Of Texas Health Science Center | |
Peterson, Stephen |
Submitted to: Journal of Antibiotics
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 4/3/2015 Publication Date: 11/1/2015 Citation: Igarashi, Y., Gohda, F., Kadoshima, T., Fukuda, T., Hanafusa, T., Shojima, A., Nakayama, J., Bills, G.F., Peterson, S. 2015. Avellanin C, an inhibitor of quorum-sensing signaling in Staphylococcus aureus, from Hamigera ingelheimensis. Journal of Antibiotics. 68(11):707-710. Interpretive Summary: Molds produce many types of chemicals that are used to influence the behavior of other microorganisms living close to them. Well known is the case of antibiotics that kill the bacteria that may be competing for food resources in nature. In this case we isolated a protein-like compound avellacin C from the mold Hamigera ingelheimensis that was not previously known. This compound prevents the bacteria Streptococcus aureus (sometimes a serious human pathogen) from performing the co-ordinated colony activity known as “quorum-sensing” that includes film formation. Biofilm formation reduces sensitivity of the bacterium to antibiotics and other environmental stress. This finding will be of interest to academic scientists and pharmaceutical researchers. Technical Abstract: Hamigera is one of the least studied genera of Eurotiales in terms of secondary metabolism compared with metabolically prolific genera such as Penicillium, Aspergillus, Paecilomyces, Monascus and Talaromyces.1 Although thousands of metabolites are known from Aspergillus and Penicillium,2 only 20–30 compounds have been reported from Hamigera.3 In our previous assessment of chemodiversity in representative strains of Hamigera, cyclic peptides known as avellanins and PF1171 were found to be widely produced by a range of this species.4 Meanwhile, one of the studied strains of H. ingelheimensis was observed to be producing an unknown congener, designated avellanin C (1) instead of the known cyclic peptides (Figure 1). In this paper, we describe the isolation and structure determination of 1 along with its quorum-sensing inhibitory activity. |