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United States Department of Agriculture

Agricultural Research Service

Research Project: Multidisciplinary Approaches to Enhanced Sugar Beet Germplasm

Location: Sugarbeet Research

Title: Identification of a SNP marker associated with WB242 nematode resistance in sugar beet

Authors
item Stevanato, P -
item Richardson, Kelley
item Panella, Leonard
item Broccanello, C -
item Saccomani, M -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 30, 2013
Publication Date: January 15, 2014
Citation: Stevanato, P., Richardson, K.L., Panella, L.W., Broccanello, C., Saccomani, M. 2014. Identification of a SNP marker associated with WB242 nematode resistance in sugar beet. Meeting Abstract. The Plant and Animal Genome XXII Conference. January 11-15, 2014. San Diego, CA.

Technical Abstract: The beet-cyst nematode (Heterodera schachtii Schmidt) is one of the major diseases of sugar beet. The identification of molecular markers associated to the nematode resistance would be helpful for developing resistant varieties. The aim of this study was the identification of SNP (Single Nucleotide Polymorphism) markers for the nematode resistant source WB242. A WB242-F2 population was phenotyped for nematode resistance. DNA of the ten most resistant and ten most susceptible individuals was pooled and subjected to Restriction-site Associated DNA Sequencing (RAD-Seq) which generated 7,241 single nucleotide polymorphisms (SNPs). Then, a subset of 384 SNPs were genotyped on 192 F2 individuals to identify SNPs associated with nematode resistance. SNP_192, localized on chromosome 5, resulted as the most significantly associated SNP (P = 4.6 × 10-13). This association was further validated on a set of resistant and susceptible varieties. We conclude that SNP_192 is a valuable marker for improvement of nematode resistance through marker-assisted selection.

Last Modified: 12/20/2014
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