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United States Department of Agriculture

Agricultural Research Service

Research Project: COTTON GENETIC RESOURCE MANAGEMENT Title: Mass spectrometry imaging of mature cotton embryos with altered seed oil and protein reserves from diverse cotton (Gossypium sp.) genotypes

Authors
item Sturtevant, Drew -
item Horn, Patrick -
item Hinze, Lori
item Percy, Richard
item Chapman, Kent -

Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 7, 2013
Publication Date: N/A

Technical Abstract: The domestication and breeding of cotton for elite, high-fiber cultivars has directly led to reduced genetic variation of seed constituents within currently cultivated accessions. A large screen of cottonseed embryos was carried out using time-domain 1H nuclear magnetic resonance (TD-NMR) for altered seed oil and protein content from the genetically diverse U.S. Cotton (Gossypium sp.) Germplasm Collection. Several genotypes within this screen showed substantial differences in oil and protein reserves that could reflect altered seed metabolism as a result of genetically different backgrounds. Recently, matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS) imaging approaches have been able to show heterogeneity within membrane (i.e. phosphatidylcholine) and storage lipids (i.e. triacylglycerols) of an upland cotton variety (Gossypium hirsutum, cv Coker 312). In this study, shotgun lipidomics of total lipid extracts and MS imaging from individual seeds with altered protein and seed oil content were used to evaluate for potential modifications in seed lipid metabolism. The results will expand our understanding of the evolution of biochemical pathways leading to the production and utilization of both storage and membrane lipids in cotton embryos. Differences in selected genotypes also will likely lead to further considerations for modification of these seed traits through breeding strategies and/or metabolic engineering of current cultivars.

Last Modified: 10/24/2014
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