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United States Department of Agriculture

Agricultural Research Service

Research Project: Epidemiology and Management of Pierce's Disease and Other Maladies of Grape

Location: Crop Diseases, Pests and Genetics

Title: Scanning electron microscopy of “Candidatus Liberibacter solanacearum” in infected tomato phloem tissue

Author
item Chen, Jianchi

Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: February 1, 2013
Publication Date: June 1, 2013
Citation: Chen, J. 2013. Scanning electron microscopy of “Candidatus Liberibacter solanacearum” in infected tomato phloem tissue. Meeting Proceedings. p. 175-178.

Interpretive Summary: “Candidatus Liberibacter solanacearum” is a putative bacterial pathogen of potato zebra-chip disease threatening the potato industry in the United States and other countries. Currently, the bacterium cannot be cultured in artificial media. Little is known about the bacterial biology. In this study, “Ca. L. solanacearum” was enriched in tomato plants and examined under scanning electron microscope. Rod, spherical, and variable shapes of cells were found. This is the first description of the multiple shapes of the bacterium. The new information expands current knowledge about this bacterium and will facilitate future research efforts.

Technical Abstract: “Candidatus Liberibacter solanacearum” is an alpha-proteobacterium associated with potato zebra-chip disease. The bacterium is currently non-cultureable. Very little is known about the bacterial morphology, an important characteristic of a complete bacterial description. In this study, “Ca. L. solanacearum” was examined by scanning electron microscopy in tomato phloem tissues where the bacterial cells were enriched. Bacillus, coccus, and pleomorphic bacterial cells were observed in phloem cells of infected tomato petioles. A preliminary analysis suggested that the different morphological types might reflect various life stages of “Ca. L. solanacearum”. The new information increases our current understanding of the biology of “Ca. L. solanacearum” and will facilitate future efforts in the bacterial cultivation and host-bacterium interaction research.

Last Modified: 10/23/2014
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