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United States Department of Agriculture

Agricultural Research Service

Research Project: TOWARD CONTROL STRATEGIES OF EMERGING PATHOGENS AND NEMATODES OF COTTON Title: Participation of chitin-binding peroxidase isoforms in the wilt pathogenesis of cotton

Authors
item Pshenichonov, Egor -
item Khashimova, Nigora -
item Akhunov, Alik -
item Golunbenko, Zamira -
item Stipanovic, Robert

Submitted to: American Journal of Plant Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 30, 2010
Publication Date: March 1, 2011
Citation: Pshenichonov, E., Khashimova, N., Akhunov, A., Golunbenko, Z., Stipanovic, R.D. 2011. Participation of chitin-binding peroxidase isoforms in the wilt pathogenesis of cotton. American Journal of Plant Science. 2:43-49.

Interpretive Summary: Plant pathogens cause significant losses to cotton production. One of the defenses used by cotton to defend itself is the production of so called enzymes that stimulate formation of barriers within the plant to wall off the pathogen propagules. Some of the enzymes involved in this process are called peroxidases. We have shown that these peroxidase enzymes accumulate more rapidly in resistant plants. This rapid increase in peroxidase activity could be developed into a useful tool that could be used by breeders to identify plants resistant to pathogens.

Technical Abstract: Specific chitin-binding isozymes of peroxidase (POX) play an important role in pathogenesis of plant diseases caused with fungi. We studied the dynamics of peroxidase activity in two varieties of cotton (Gossypium hirsutum L.); one was a susceptible and the other resistant to the plant pathogen Verticillium dahliae. After infection with a strongly and a weakly virulent isolate of V. dahliae, we observed a correlation between the level of seedling tissue lesion and peroxidase activity. Thus, the first POX activity was observed in all infected plants 2 hours after inoculation, but POX activity of the resistant variety rapidly increased and maximized 3 days after infection, while POX activity in the susceptible variety showed a slow increase that continued to increase during the remaining 8 days of experimental observation. The increase of POX activity in the susceptible variety after infection may be explained by progressive fungal colonization of cotton tissues leading to irreversible senescence. Microscopic examination of plant tissues supports this hypothesis. The more virulent isolate caused more necrosis and significantly more POX activity than the mildly virulent in both susceptible and resistant plants. Control plants showed no changes in POX activity; however, the POX activity in the control resistant varieties was higher than the control susceptible varieties. These findings indicate the potential utilization of chitin binding POX as a biochemical tool to guide breeding programs to increase resistance to V. dahliae.

Last Modified: 4/21/2014
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