Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: March 12, 2010
Publication Date: March 16, 2010
Citation: Bakst, M.R. 2010. Chapter II. Determination of sperm concentration. Section 1. Hemocytometer procedure. In: Bakst, M.R., Long, J.A., editors. Techniques for Semen Evaluation, Semen Storage, and Fertility Determination. 2nd edition. St. Paul, MN: The Midwest Poultry Federation. p. 11-15.
Interpretive Summary: Determining the sperm numbers in a semen sample gives a measure of semen quality and is necessary if the semen is to be inseminated based on the numbers of sperm per insemination. The hemocytometer allows the investigator to could the number of cells directly from a grid. To use instruments determining sperm concentration, one must calibrate a given reading for that instrument to actual sperm numbers. This task is described in another chapter. The third chapter describes the use of one such instrument and how to derive actual sperm numbers. The chapters are geared toward both poultry industry and poultry research personnel.