Technical Abstract: Honey bee viruses display a host range that is not restricted to their original host, European honey bees, Apis mellifera. Here we provide the first evidence that Black Queen Cell Virus (BQCV), one of the most prevalent honey bee viruses, can cause an infection in both laboratory-reared and field-collected bumble bees, Bombus huntii, with the infection spreading to different tissues within the bumble bees. Additionally, the infection of BQCV was more widespread in bumble bees collected from the field in comparison to laboratory-reared bees. The detection of negative strand RNA of BQCV, an indicator of active virus replication, in the gut of B. huntii suggests that the gut is a primary site of BQCV infection and that the virus particles replicate within the gut and then cross the gut lining to other tissues through hemolymph circulation. The observation of active replication of the BQCV in the gut, together with the fact that BQCV was more widespread in the body of field-collected bees than that of lab-reared bees, implies a possible association between the foraging activities of bumble bees and virus transmission. The fact that bumble bees and honey bees are able to share nectar and pollen resources in the same field suggests that geographical proximity of two host species, could play a role in host range breadth of BQCV.