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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC AND CULTURAL METHODS TO MANAGE RENIFORM NEMATODE IN COTTON Title: Identification of Pasteuria spp. that parasitize Rotylenchulus reniformis

Authors
item Hewlett, Thomas -
item Stetina, Salliana
item Schmidt, Liesbeth -
item Waters, John -
item Simmons, Lee -
item Rich, Jimmy -

Submitted to: Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: July 15, 2009
Publication Date: December 1, 2009
Citation: Hewlett, T.E., Stetina, S.R., Schmidt, L.M., Waters, J.P., Simmons, L.J., Rich, J.R. 2009. Identification of Pasteuria spp. that parasitize Rotylenchulus reniformis. Journal of Nematology. 41(4):338.

Technical Abstract: Reniform nematodes (Rotylenchulus reniformis) collected from cotton research plots in Quincy, Florida, a commercial field in Huxford, Alabama, and multiple west central Mississippi cotton farms were observed to have Pasteuria-like spores attached to their cuticles. Reniform nematodes with spores attached from the Huxford, Alabama sample were surface sterilized, ruptured and endospores were propagated in-vitro by Pasteuria Bioscience, INC. Endospores produced from this inoculum were attached to uninfected reniform nematodes and incubated in sterilized soil at 29.5 C. Spore-filled reniform nematodes were recovered from the soil after 10 days. Genomic DNA extracts were prepared from spores recovered from the infected cadavers. PCR using degenerate primers provided partial coding sequences for spoIIAB, atpA, atpF, and 16s rDNA, having significant homology to Pasteuria spp., thus establishing the first reported infection and in-vitro propagation of Pasteuria spp. for the reniform nematode.

Last Modified: 10/22/2014
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